Alesaia A. Tietze Research 2

Research Methods

Peptide Chemistry and Biology:

  • Peptide synthesis of linear and cyclic peptides
  • Targets compounds: amino acid derivatives, short, medium-sized and long peptides, miniproteins, peptide libraries modified peptides (PTMs), peptide/protein conjugates
  • Peptide analytics: analytical and semipreparative HPLC (RP, IEC), TLC, amino acid analysis (AAA),
  • Peptide structure elucidation: CD, solution NMR

Biochemistry/Protein biochemistry/Proteomics/Molecular Biology:

  • Protein-protein and protein-ligand interactions
  • Recombinant proteins: primer design, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western Blot, real time polymerase chain reaction, agarose-gel electrophoresis, DNA/RNA isolation/purification, plasmid sub-cloning, restriction enzyme digestion, transformation of competent E. coli cells, transfection, genome sequencing und assignment, protein isolation/quantification, affinity chromatography (Ni-NTA, amylose), Coomassie-staining, silver-staining, cell culture
  • Mass spectrometry of peptides and proteins: LC-ESI MS, MALDI-TOF/TOF MS

Bioinformatics

  • Molecular modeling
  • Generation and optimization of homology models
  • Computational binding studies (Docking)
  • Molecular simulation studies