Development of New Biocatalytic Syntheses
We are employing native and recombinant enzymes to synthesize bioactive natural products as well as structurally related, non-natural mimetics that may be potentially bioactive. A pivotal aspect is the utilization of enzymes as the only source of chirality for asymmetric induction. Major efforts are concerned with biocatalytic asymmetric CC bond formations, particularly employing enzymes from carbohydrate metabolism.
Development of New Enzymatic Catalysts
Overproduction of novel enzymes by recombinant DNA technology provides an important basis for the identification of synthetically useful biocatalysts and for the development of novel and highly stereocontrolled routes for the synthesis of carbohydrates and glycoconjugates.
Broadening the Scope for Applications of Known Enzymes
We are studying the catalytic mechanisms of enzymes using methods of enzymology and X-ray structure determination (colaborative work with protein crystallographers) to facilitate the rational alteration of substrate binding residues by site-directed mutagenesis. Goals are improved substrate tolerance, stereoselectivity, and stability for synthetically useful enzymes.
