Potent inhibitors of human matriptase derived from MCoTI-II Variants.|
Bernhard Glotzbach, Niklas Weber, Michael Tomaszowski, Björn Hock, Harald Kolmar
Ref. No: EP13001869.0., Year: 05/2013
HIGH-AFFINITY MATRIPTASE INHIBITORS|
Fittler H, Empting M, Glotzbach B, Kolmar H, Ref. No: EP13000310.6, Year: 01/2013
GENERAL STRATEGY FOR ANTIBODY LIBRARY SCREENING|
S Becker, T Heiseler, A Schuhmacher, H Kolmar, Ref. No: EP11009901.7, Year: 01/2012
ABSTRACT: A generally applicable method for the selective covalent attachment of a reporter molecule to a replicating entity that allows one to obtain specific binders from a single round of library screening is disclosed. For example, selective biotinylation of phage particles and yeast cells displaying a binder to any given target can be achieved via application of a coupled enzyme reaction that includes a peroxidase, an oxidase and a catalase.
POLYPEPTIDE COMPRISING A KNOTTIN PROTEIN MOIETY|
M Blind, H Kolmar, Ref. No: WO2008098796, Year: 08/2008
ABSTRACT: The present invention is related to a polypeptide comprising a scaffold moiety and a helix moiety, whereby the helix moiety is inserted into the scaffold moiety, the scaffold moiety comprises a knottin protein or at least one fragment thereof, and the amino acid sequence of the polypeptide differs from the amino acid sequence of the knottin protein or at least one fragment thereof.
MEANS AND METHODS FOR DISPLAYING POLYPEPTIDES ON CELLS, T Adams, H Kolmar, Ref. No: MEANS AND METHODS FOR DISPLAYING POLYPEPTIDES ON CELLS, Year: 01/2007 |
Abstract: Described is a method for presenting a polypeptide of interest on the surface of cells comprising growing and partially lysing cells which express said polypeptide of interest. Also described are methods for identifying cell clones expressing a polypeptide with a desired property and methods for identifying the nucleotide sequences encoding such a polypeptide. Furthermore, gram-negative bacterial cells are described which contain genetic information for expressing the outer membrane protein and intimin and optionally for expressing a protein of interest.
USE OF MICROPROTEINS AS TRYPTASE INHIBITORS|
H Kolmar, C Sommerhoff, A Wentzel, Ref. No: WO2006032436, Year: 03/2006
Abstract: Disclosed are uses of microproteins preferably microproteins forming a cystine knot (i.e. belonging to the family of inhibitor cystine knot (ICK) polypeptides) or polynucleotides encoding said microproteins for the preparation of a pharmaceutical composition for treating or preventing a disease that can be treated or prevented by inhibiting the activity of tryptase as well as corresponding methods of treatment. Also disclosed are uses of the microproteins for inhibiting tryptase activity, for purifying tryptase, as a carrier molecule for tryptase and for deleting or quantifying tryptase in a sample, including corresponding diagnostic applications. Furthermore disclosed are fusion proteins comprising an inactive barnase as well as fusion proteins comprising barnase and a microprotein. Also encompassed are nucleic acid molecules encoding such a fusion protein, as well as corresponding vectors, host cells, preparation methods and uses of the fusion protein. Moreover, the present application discloses a crystal of a microprotein fused with barnase, preferably inactive barnase. The disclosure also refers to corresponding preparation methods for the crystal, structure analysis methods using the crystal data storage media comprising the structure data obtained, as well as to in silico methods using the structure data for characterizing the binding of microproteins to target molecules. Furthermore, disclosed are pharmaceutical compositions comprising the crystal and corresponding medical uses.
Dimeric or Multimeric Microproteins|
A Wentzel, E Boehnlein, H Kolmar, HU Schmoldt, Ref. No: WO2006094813, Year: 03/2006
Abstract: Disclosed is a polypeptide comprising at least two microproteins, which preferably comprise an amino acid sequence having a specific binding activity to a target protein. Furthermore, disclosed are polynucleotides encoding such a polypeptide as well as pharmaceutical compositions and kits comprising said polypeptide or polynucleotide. Also disclosed herein are methods of treatments and second medical uses applying the disclosed polypeptide or polynucleotide. Additionally, the disclosure of the present application relates to a method for forming a covalent bond in a microprotein which can be used for producing the disclosed polypeptides.
METHOD FOR THE IDENTIFICATION OF ENZYMES HAVING DESIRED PROPERTIES BY IMMOBILISATION OF THE REACTION PRODUCT ON THE SURFACE OF ENZYME-PRESENTING ORGANISMS|
H Kolmar, Ref. No: WO2005040408, Year: 06/2005
Abstract: The invention relates to a method for the identification of enzymes with a desired activity, by random generation of a large collection of enzyme variants, the synthesis of said variants in host organisms, the presentation thereof on the surface of the organisms and the isolation of enzyme variants with the desired properties, by the detection of the covalent deposition of the reaction product on the surface of the host organism.
METHOD FOR EXPOSING PEPTIDES AND POLYPEPTIDES ON THE CELL SURFACE OF BACTERIA|
H Kolmar, A Christmann, A Wentzel, Ref. No: WO2002034906, Year: 01/2002
Abstract: The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E.coli cell membrane. According to said method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.
Genetic selection of proteins able to bind a ligand by signal-transduction in a microorganism|
H-J Fritz, F Hennecke, H Kolmar, Ref. No: EP0630968, Year: 06/2002
Abstract: The invention relates to a method for the genetic selection of proteins able to bind a ligand in microorganisms, characterised in that a protein able to bind a ligand is presented extracytoplasmically, and the signal of ligand binding is passed on by signal transduction to the biosynthetic machinery of the microorganism for expression of a detectable and/or selectable function. Also disclosed are microorganisms and replicas suitable for this method and processes for the preparation thereof.