Strychnine is something like the white mouse of a NMR spectroscopist. The proton spectrum of strychnine shows almost everything that can be imagined: Chemical shifts anywhere between 0 to 10 ppm, strong couplings, diastereotopous protons, interesting multiplicities, wide and narrow lines. Many pulse sequences are therefore tested at strychnine.

Therefore, strychnine was also the test molecule for our first RDC investigations. In a “proof-of-principle”, we have shown that all diastereotopous protons in strychnine can be clearly assigned by RDCs without the use of NOEs.

Coworker on that area

1 C. M. Thiele, S. Berger, Probing the Diastereotopicity of Methylene Protons in Strychnine Using Residual Dipolar Couplings, Org. Lett. 2003, 5, 705-708.
2 C. M. Thiele, Poly-γ-ethyl-Lglutamate as Alignment Medium for the Measurement of RDCs in Organic Substrates, J. Org. Chem. 2004, 69, 7403-7413.