Peptides can be considered as small proteins or heteropolymers consisting of amino acids as building blocks. Classical peptides are assembled by means of organic chemistry methods from natural α-L-amino acids. For special research (in order to obtain alignment media) other unnatural building blocks can be used.
Being at least bifunctional, by bearing amino- and carboxy-functionalities, amino acids require to be protected by so-called temporary protecting groups for every coupling stage. Other potentially reactive side chain groups are usually blocked semi-permanently. Thus, the whole general procedure is as follows:
- Synthesis of selectively protected amino acids or other building blocks
- Stepwise assembling of peptide from building blocks in solution or on solid phase. The latter can also be performed by an automatic synthesizer:
- Elaboration of condensation reaction.
- Deprotection of temporary blocking group.
- After a number of necessary steps of condensation and deprotection, the final stage of complete deprotection, i. e. getting rid of semi-permanent blocking group, gives us the crude peptide.
- Peptide purification by chromatography methods.
- Chemical structure confirmation.
Our group is equipped with a peptide synthesizer (Liberty, CEM, USA) coupled with a microwave-generator (Discover, CEM, USA) and a HPLC instrument (JASCO, Japan). We perform peptide synthesis at all stages described above, from amino acid derivatives to final peptides (see beta-peptides).
Coworker on that area
|Dr.-Ing. Witali Blum|
|Dr. Yulia Moskalenko|