Instrumentation & Equipment

Below you will find a list of the instrumentation and equipment available in the Thiele lab and a short description of their respective applications.

NMR spectrometers

The focus in our group lies on research using NMR (Nuclear Magnetic Resonance) spectroscopy. In our NMR facility, which was inaugurated in 2013, three NMR spectrometers are set up to be used for a wide range of tasks.

Our 400 MHz spectrometer (Bruker Avance III HD) is equipped with an autosampler so that a large number of samples can be measured automatically one after the other. Four probes are available for this device, with which 5 or 10 mm samples can be measured. Applications range from high temperature measurements (up to ~480 K) of polymer melts, diffusion measurements of polymer solutions, imaging (via 1H, 19F or 2H) to the measurement of general NMR data sets (including 1H decoupled 19F spectroscopy and multidimensional spectra).

Our 600 MHz instrument (Bruker Avance III) is equipped with a triple resonance broadband inverse probe (TBI; 1H/31P, BB/2H) as default, which can be used to measure a wide range of homo- and heteronuclear experiments or diffusion of small molecules in a temperature range between 240 K and 350 K, among other things. A nitrogen evaporator is also available for the device, so that low-temperature measurements down to 180 K can be carried out. Furthermore, an HR-MAS probe (1H, 13C, 2H) is available for this spectrometer, which can be used to perform measurements of anisotropic highly viscous gels, liquid crystalline phases and (to a limited degree) solids.

The 700 MHz spectrometer (Bruker Avance III HD) is equipped with a helium-cooled cryoprobe (quadruple resonance inverse probe; 1H/19F, 31P/13C/15N/2H), with a significantly increased sensitivity for measurements on samples with low concentrations or higher-dimensional homo- and heteronuclear correlation experiments.

UV/Vis und IR spectrometer

Apart from NMR spectroscopy, the Thiele lab has other analytical instruments at its disposal. UV/Vis spectra can be measured using a Jasco spectrophotometer or an Avantes UV/Vis fibre spectrometer.

Infrared spectra are measured on a Bruker Alpha spectrometer equipped with an ATR unit and a transmission unit.

The two available measuring modules allow for the measurement of pure compounds in solid or liquid form as well as in solution, as Nujol mull or in KBr discs. The Bruker Alpha is so small that it can easily be brought into our glovebox, so that measurements of air-sensitive compounds under argon atmosphere are also possible.

GPC and HPLC

Furthermore, we have a gel-permeation chromatography (GPC) setup for the analysis of the polymers produced in our laboratories. Both a UV/Vis and a refractive index detector can be used for measurements. Using appropriate columns, the GPC setup can be converted to an HPLC (high-performance liquid chromatography) setup.

Glovebox

A glovebox is essential for the preparation and synthesis of air and water-sensitive substances. Argon is used as the inert gas, allowing for demanding syntheses Provided with a large antechamber, analytical equipment may also be brought into the box.

Photoreactor

We have a photoreactor for special photochemical reactions. This instrument is equipped with a cooling tube and a reaction tube. This allows the continuous production of products of photochemical reactions

grin flow-through mode.

Lyophilizer

lyophilizer

Our lyophilizer enables gentle drying of synthetic products. This is necessary, for example, for thermally labile substances. This process is often used for the purification of peptides.